Journal: medRxiv
Article Title: Expression of mechano-growth factor (MGF) in refractory overactive bladder
doi: 10.1101/2023.12.08.23299594
Figure Lengend Snippet: An antibody specific to the MGF E-peptide was used to detect expression of MGF protein by immunohistochemistry staining (a) and western blot (b-d) analysis of biopsy sections. In (a) 5µm sections of formalin-fixed OAB bladder biopsies were stained with anti-MGF (i) or secondary antibody only control (ii) and counterstained with haematoxylin. In (b-d) 30µg biopsy lysates were analysed by western blot for detection of MGF (∼15 kDa) and β-actin (42 kDa) as a loading control. L=size ladder. A representative blot is shown in (b) and a scatter plot showing quantification of MGF band intensity is shown in (c). The bar indicates mean relative MGF expression in control and OAB biopsies (0.22 and 0.51, respectively). In (d), the graph shows mean relative MGF expression +/- SEM in control/OAB biopsies in the three main sub-groups identified in : DES hi (n=6/5), VIM hi (n=1/6), DES&VIM hi (n=2/6). Statistical analysis in (c) and (d) was performed using the Mann-Whitney U test.
Article Snippet: Sections were stained using an anti-MGF E-peptide antibody (Merck Life Sciences #07-2108, rabbit polyclonal, 1/50 dilution) and HRP-tagged secondary (Vector Laboratories, BA-1100, 1/200 dilution), or secondary only control staining.
Techniques: Expressing, Immunohistochemistry, Staining, Western Blot, MANN-WHITNEY